Respuesta inmune Th1/Th2 en pacientes co-infectados con Helicobacter pylori y helmintos / Immune response Th1/Th2 to Helicobacter pylori and Helminths in co-infected patients

Resumen: Introducción: La inflamación asociada con la infección por Helicobacter pylori (H. pylori) se relaciona con la pro gresión de las lesiones precancerosas gástricas. Las infecciones por helmintos podrían modular la respuesta proinflamatoria a la infección por H. pylori desde un perfil tipo LTCD4+ Th1 hacia una respuesta menos perjudicial tipo LTCD4+ Th2. Objetivo: Caracterizar la polarización de la respuesta inmune tipo LTCD4+ Th1/Th2 de pacientes coinfectados por H. pylori y helmintiasis procedentes de áreas de bajo riego para el desarrollo de cáncer gástrico. Pacientes y Método: Se analizaron 63 pacientes, 40 adultos y 23 niños infectados con H. pylori. La determinación de los perfiles séricos de las interleucinas asociadas con la polarización de la respuesta inmune tipo LTCD4+ Th1 (IL-1Β, INF-γ y TNF-α) y tipo LTCD4+ Th2 (IL-4, IL-10 e IL-13) se realizó con Análisis Multiplex (xMAP). La relación entre el estado de coinfección por helmintos en pacientes infectados con H. pylori y la polarización de la respuesta inmune mediada por LTCD4+ Th1 y LTCD4+ Th2, se estudió con un modelo de regresión logístico de efectos mixtos. Resultados: La frecuencia de helmintos fue similar en adultos (15%) y niños (17%). La polarización de la respuesta inmune fue más prevalente hacia el tipo LTCD4+ Th1. Los valores séricos de las interleucinas asociadas con la polarización de la respuesta inmune tipo LTCD4+ Th1 (IL-1 Β, INF-γ y TNF-α) y tipo LTCD4+ Th2 (IL-4, IL-10 e IL-13) fueron independientes del estado de infestación por helmintos. Conclusión: La prevalencia de infección por parasitismo intestinal fue alta y la polarización de la respuesta inmune fue predominantemente hacia un perfil tipo LTCD4 + Th1.

Abstract: Introduction: Inflammation associated with Helicobacter pylori (H. pylori) infection is linked to the development of a gastric precancerous lesion. Helminth infections could influence the pro-inflam matory response to such infection from LTCD4+ Th1 to a less harmful LTCD4+ Th2 response. Ob jective: To characterize the polarization of the LTCD4+ Th2 immune response in co-infected pa tients with H. pylori and helminths from low-risk areas for developing gastric cancer. Patients and Method: We analyzed 63 patients infected by H. pylori (40 adults and 23 children). Through the Multiplex Analysis technology (xMAP), we determined the serum profiles of the interleukins asso ciated with the polarization of the immune response of LTCD4+ Th1 (IL-1Β, INF-γ, TNF-α) as well as the LTCD4+ Th2 (IL-4, IL-10, and IL-13). The ratio between helminths co-infection status in H. pylori-infected patients and the polarization of the immune response mediated by LTCD4+ Th1 and LTCD4+ Th2 was assessed using a Mixed Effects Logistic Regression Model. Results: The frequency of helminths was similar between adults (15%) and children (17%). The polarization of the immu ne response was more prevalent in LTCD4+ Th1. Serum values of interleukins associated with the immune response polarization of LTCD4+ Th1 (IL-1Β, INF-γ, and TNF-α) and LTCD4+ Th2 (IL-4, IL-10, and IL-13) were independent of helminths infection status. Conclusion: The prevalence of in testinal parasitic infection was high and the immune response polarization was mainly LTCD4 + Th1.

Total IgE level in respiratory allergy: study of patients at high risk for helminthic infection

OBJETIVO: Eosinofilia e elevação de IgE sérica são expressões de atopia, contudo há fatores intervenientes como, por exemplo, as parasitoses intestinais. Esta pesquisa verifica a relação entre IgE sérica total, eosinófilos e IgE específica anti-áscaris em indivíduos portadores de asma e/ou rinite alérgica. MÉTODOS: Estudo do tipo transversal em adolescentes portadores de asma e/ou rinite alérgica que foram examinados quanto ao nível sérico de IgE total, de IgE anti-áscaris e de contagem dos eosinófilos sangüíneos. RESULTADOS: Foram analisados 101 pacientes com idade entre 12 e 21 anos. A mediana da IgE foi 660 UI/mL (P25-75 243,5-1500), e a dos eosinófilos foi 510 células/mm³ (P25-75 284-811). A IgE anti-áscaris foi positiva em 73 por cento (74/101) da amostra, mas houve apenas 33,7 por cento (34/101) de positividade ao parasitológico de fezes. Os coeficientes de correlação encontrados foram: 0,34 (p = 0,001) entre IgE total e eosinófilos, 0,52 (p < 0,001) entre IgE total e IgE anti-áscaris e 0,26 (p = 0,01) entre eosinófilos e IgE anti-áscaris. O modelo de regressão linear múltipla final encontrou que IgE anti-áscaris foi fator contribuinte para IgE sérica total com coeficiente de determinação (r2 ajustado) de 0,25 (F = 12,35; p < 0,001), e esse efeito foi independente de contagem de eosinófilos séricos e de parasitose intestinal por helmintos. CONCLUSÃO: Em pacientes com alergia respiratória e IgE sérica total bastante elevada, oriundos de áreas com alto risco de infecção por helmintos, a pesquisa de IgE anti-áscaris como possível fator explicativo pode ser de maior ajuda que a realização do parasitológico de fezes.

OBJECTIVE: Eosinophilia and increased serum IgE levels are indicators of atopy; however, other factors can also play a key role, such as intestinal parasitic infections. This study assesses the relationship between total serum IgE, eosinophil count, and anti-Ascaris IgE in individuals with asthma and/or allergic rhinitis. METHODS: A cross-sectional study was carried out in adolescents with asthma and/or allergic rhinitis. The patients had their total serum IgE, anti-Ascaris IgE and eosinophil count measured. RESULTS: A total of 101 patients aged 12 to 21 years were assessed. Median IgE level was 660 IU/mL (P25-75 243.5-1500), and the eosinophil count corresponded to 510 cells/mm³ (P25-75 284-811). Anti-Ascaris IgE was positive in 73 percent (74/101) of the individuals, but parasitological stool examination yielded positive results in only 33.7 percent (34/101). The correlation coefficients were the following: 0.34 (p = 0.001) between total IgE level and eosinophil count, 0.52 (p < 0.001) between total IgE level and anti-Ascaris IgE, and 0.26 (p = 0.01) between eosinophil count and anti-Ascaris IgE. The final multiple linear regression model pointed out that anti-Ascaris IgE contributed to a total serum IgE level with a coefficient of determination (adjusted R²) of 0.25 (F = 12.35; p < 0.001). This effect occurred regardless of eosinophil count and of the presence of intestinal helminthic infection. CONCLUSION: In patients with respiratory allergy and increased total serum IgE levels living in areas where there is a high risk for helminthic infections, the quantification of anti-Ascaris IgE can be more useful and more insightful than the parasitological stool examination.

Inmunodiagnóstico de las infecciones por Strongloides stercoralis en Chile utilizando la prueba de ELISA / Immunodiagnosis of Strongyloides stercoralis infections in Chile using ELISA test

BACKGROUND: Strongyloides stercoralis is a world wide distributed small intestinal nematode parasite. In immunocompetent individuals S stercoralis can produce asymptomatic infections or a moderate clinical picture of diarrhea, some cases become chronic. In immunocompromised patients, a disseminated disease may appear, sometimes fatal. In Chile, there is little epidemiological information about S stercoralis infections and appropriate diagnostic techniques are usually not used. AIM: To evaluate the yield of an ELISA test for the diagnosis of strongyloidiasis in Chilean patients. MATERIAL AND METHODS: Ten serum samples from patients with S stercoralis infections confirmed by a positive stool examination, 66 samples from individuals with other infections by tissue helminthes (24 toxocariasis, 15 trichinellosis, 11 hydatidosis, 12 fascioliasis and 4 cysticercosis), 13 samples from subjects with autoimmune diseases and 49 samples from apparently healthy individuals with a normal eosinophil count, were studied. ELISA antigen was prepared using a filariform larval extract obtained from a murine species of Strongyloides, maintained in laboratory animals. RESULTS: Using 0.33 optical density units as a cut off value, 9 of 10 sera of S stercoralis infected individuals, had a positive ELISA test. No cross reactions were observed with sera of patients with other helminthic infections, autoimmune diseases or in healthy individuals. Thus, specificity, positive and negative predictive values were 100 per cent. CONCLUSIONS: The results obtained are similar with those found by other investigators. ELISA test for strongyloidiasis is a useful tool for the diagnosis of clinical cases and for seroepidemiological studies of this nematode infection in Chile.

Eosinofilia devida a parasitas / Eosinophilia due to parasites

Eosinófilos estäo envolvidos em uma série de infecçöes parasitárias mas, nem sempre o número destes está aumentando no sangue circulante. Considera-se nesta breve revisäo alguns dos aspectos envolvidos na produçäo e funçäo destas células em parasitoses causadas por nematódeos, cestódeos, trematódeos e protozoários mais comuns no Brasil. Normalmente ocorre eosinofilia em associaçäo à infecçäo helmíntica, na qual há envolvimento de mucosa. Nos casos de protozooses, a eosinofilia é, praticamente, ausente

Prevalence of anaemia and worm infestation in tribal areas of Madhya Pradesh.

The study was conducted among school going children (6-14 years) of Baiga, Abuihmadia and Bharia tribes of Madhya Pradesh to assess the prevalence of anaemia and intestinal parasitic infestation among themselves. A total of 776 school going children were included in the study of whom blood samples of all and stool samples of 409 were collected. Their haemoglobin was measured and stool samples were examined under microscope for ova and cysts. The results revealed that 30.3% of the children had severe anaemia (Hb < 7g/dl) and 50% children had intestinal parasites. The most common parasites were hookworn (16.3%) and A lumbricoides (18.5%). Though hookworm ova loads indicated mild to moderate infestation in most of the children, the continued presence of worms in marginally nourished children could contribute significantly to blood loss in the intestine with resultant anaemia.

Serología para detección de helmitos extraintestinales en niños con alteración neurológica / Serology for detection of extraintestinal helminthes in children with neurological disorders

Se estudiaron los sueros de 207 niños, con edades desde 20 meses hasta 17 años 11 meses, con diagnóstico clínico de parálisis cerebral infantil (PCI), de un centro de rehabilitación neurológica en la Ciudad de México, D.F. Objetivo. Detectar reactividad serológica contra antígenos de helmintos con localización extraintestinal, en niños con alteraciones neurológicas. Los antígenos utilizados se obtuvieron de: el metacéstodo de Taenia solium (cisticercos); Fasciola hepatica, Toxocara canis, Ascaris lumbricoides lumbricoides y Trichinella spiralis. Como prueba inmunológica se utilizó la hemaglutinación indirecta; se consideró como título de reactividad positivo la dilución 1:32 o mayor. Diecisiete (8.2 por ciento) de los sueros resultaron con títulos positivos a los siguientes antígenos: 4 (1.9 por ciento) al metacéstodo de T. solium, 1 (0.5 por ciento) a F. hepatica, 4 (1.9 por ciento) a T. canis, 4 (1.9 por ciento) a A. I. lumbricoides y 4 (1.9 por ciento) a T. spiralis. Con base en los resultados obtenidos se hacen recomendaciones al personal médico, paramédico y familiar de extremar las precauciones en la atención y cuidado de los niños con deficiencia motora

Anthelminthic treatment raises plasma iron levels but does not decrease the acute-phase response in Jakarta school children.

The study was conducted to investigate the impact of intestinal helminthiasis and treatment on iron status and acute phase response (APR) among urban Indonesian primary school children, aged 8-11 years old. The prevalence of helminthiasis among these children was; Ascaris lumbricoides, 81.6%; Trichuris trichiura, 88.3%; and mixed infection of A. lumbricoides and T. trichiura, 70.0%. Of 120 children enrolled in the investigation, 59 received a single 400 mg dose of albendazole, and 61 received a placebo. Ten days following treatment, the prevalence of ascariasis and trichuriasis in the treatment group diminished to 0% and 27%, respectively, and in the placebo group to 63.9% and 68.9%. Plasma iron, hemoglobin, C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), white blood cell (WBC), interleukin-1 (IL-1), interleukin-6 (IL-6) and tumor necrosis factor (TNF) concentrations were determined prior to the intervention and 10 days after. Plasma iron concentrations and WBC count rose in the treatment group (p=< or =0.05) when compared to baseline status. Increases in hemoglobin concentrations observed in the treatment group 10 days post-treatment were not statistically significant. CRP, IL-1, IL-6 and TNF were found to be within normal limits for both groups both before and after treatment. ESR increased significantly in both treatment and placebo groups when compared the rates measured before treatment. These findings show that treatment with albendazole is associated not only with a decreased worm burden in school children, but also a rise in plasma iron.

Prevalence of intestinal parasites in a rural community in north-central Puerto Rico

Se realizó un estudio hematológico y parasitológico en Bayaney, Hatillo, una comunidad rural localizada en la parte norte-central de Puerto Rico. Un hallazgo sorprendente fue la alta proporción comparativa de casos de eosinofilia encontrados (43 de 83; 52%). De estos 83 casos, 16 resultaron positivos para parásitos intestinales utilizando el método de concentración (Formol-Ether) Ritchie. Con eosinofilia de 5 a 10%, sen encontró infección con un parásito intestinal en 35% de los casos, con eosinofilia mayor de 10%, el 75% de los casos estaban infectados con un parásito intestinal. Los parásitos o larvas encontrados fueron: Uncinaria (N=8), Stronglyoides Stercoralis (N=2), Trichuris Trichiura (N=5) y un caso con una infección combinada de uncinaria y trichuris. Quince de estos 16 (94%) casos con parásitos intestinales tenían eosinofilia de 5 a 40%. De un subgrupo de niños de quinto grado de escuela, 11 de 35 (31%) tenían eosinofilia de 5 a 17%, pero solo dos de estos tenían huevos de parásitos (Trichuris). No se encontraron protozoos intestinales, a pesar de que todos los casos infectados estaban aparentemente asintomáticos, se les informó del resultado del estudio y en aquellos casos necesarios se les suplió examen médico y tratamiento. Se estudió la presencia de anticuerpos contra el huevo de S. mansoni por ELISA, usando una fracción de antígeno cationico llamado CEF-6. De 44 sueros analizados, 20 eran de un grupo de alto riesgo debido al contacto frecuente con agua infectada. Por serología solo un caso (2%) fue positivo y en este, el examen de excreta para huevos de S. mansoni fue negativo. Se necesitan estudios adicionales para determinar la etiología de eosinofilia en ausencia de infección por parásitos intestinales

Human intestinal helminthiases in East Timor.

In a study of 210 people from all age groups in the Venilale District of East Timor, 49% had Ascaris lumbricoides, 1% Trichuris trichiura and 67% hookworm infection. There were high Ascaris infection rates among some of the children, but the Trichuris and hookworm rates were almost uniformly low. The factors responsible for these rates are enumerated, and the complex interaction of the factors is discussed. The relative lack of shade and the well-drained limestone soil are probably in large measure responsible for the generally low helminth prevalence. Ascaris was the principal cause of eosinophilia. It was not possible to relate any other haematological or serological findings to the helminths. Apart from the high Ascaris infections in young children, it is not thought that these helminths constitute a heatlh problem.